Simultaneous saccharification and fermentation of amorphous cellulose to...
AbstractA derivative of Klebsiella oxytoca M5A1 containing chromosomally integrated genes for ethanol production from Zymomonas mobilis (pdc, adhB) and endoglucanase genes from Erwinia chrysanthemi...
View ArticleProduction of d(−)-lactate from sucrose and molasses
AbstractEscherichia coli W3110 derivatives, strains SZ63 and SZ85, were previously engineered to produce optically pure d(−) and l(+)-lactate from hexose and pentose sugars. To expand the substrate...
View ArticleFermentation of sugar cane bagasse hemicellulose hydrolysate to l(+)-lactic...
AbstractSugar cane bagasse hemicellulose, hydrolyzed by dilute H2SO4, supplemented with mineral salts and 0.5% corn steep liquor, was fermented to l(+)-lactic acid using a newly isolated strain of...
View ArticleGlobal gene expression analysis revealed an unsuspected deo operon under the...
AbstractModE protein, a molybdate sensor/regulator, controls the transcription of genes coding for molybdate uptake (mod), molybdopterin synthesis (moa), molybdoenzymes nitrate reductase (nap) and...
View ArticleFermentation of 10% (w/v) Sugar to D(−)-Lactate by Engineered Escherichia coli B
AbstractDerivatives of ethanologenic Escherichia coli K011 were constructed for d(−)-lactate production by deleting genes encoding competing pathways followed by metabolic evolution, a growth-based...
View ArticleFermentation of 12% (w/v) Glucose to 1.2 m Lactate by Escherichia coli Strain...
AbstractA non-recombinant mutant of Escherichia coli B, strain SZ194, was developed that produces over 1 md-lactate from glucose (or sucrose) in 72 h using mineral salts medium supplemented with 1 mm...
View ArticleBetaine Tripled the Volumetric Productivity of d(-)-lactate by Escherichia...
AbstractOsmotic stress restricts glycolytic flux, growth (rate and yield), d-lactate productivity, and d-lactate tolerance in Escherichia coli B strain SZ132 during batch fermentation in mineral salts...
View ArticleMethylglyoxal Bypass Identified as Source of Chiral Contamination in l(+) and...
AbstractTwo new strains of Escherichia coli B were engineered for the production of lactate with no detectable chiral impurity. All chiral impurities were eliminated by deleting the synthase gene...
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Development of Ethanologenic Bacteria
AbstractThe utilization of lignocellulosic biomass as a petroleum alternative faces many challenges. This work reviews recent progress in the engineering of Escherichia coli and Klebsiella oxytoca to...
View ArticleCombined effect of betaine and trehalose on osmotic tolerance of Escherichia...
AbstractIn mineral salts medium, supplementing with betaine in combination with increased production of endogenous osmoprotectant from a second copy of the trehalose biosynthetic genes (otsBA) improved...
View ArticleLow salt medium for lactate and ethanol production by recombinant Escherichia...
AbstractIndividual nutrient salts were experimentally varied to determine the minimum requirements for efficient l(+)-lactate production by recombinant strains of Escherichia coli B. Based on these...
View ArticleProduction of l-alanine by metabolically engineered Escherichia coli
AbstractEscherichia coli W was genetically engineered to produce l-alanine as the primary fermentation product from sugars by replacing the native d-lactate dehydrogenase of E. coli SZ194 with alanine...
View ArticleSucrose and overexpression of trehalose biosynthetic genes (otsBA) increase...
AbstractSurvival after desiccation was highest for recombinant strains of E. coli engineered to produce ethanol (KO11 and LY163) and lactate (TG106) when sucrose was provided as the fermentable sugar....
View ArticleRe-engineering Escherichia coli for ethanol production
AbstractA lactate producing derivative of Escherichia coli KO11, strain SZ110, was re-engineered for ethanol production by deleting genes encoding all fermentative routes for NADH and randomly...
View ArticleThermophilic Bacillus coagulans Requires Less Cellulases for Simultaneous...
AbstractEthanol production from lignocellulosic biomass depends on simultaneous saccharification of cellulose to glucose by fungal cellulases and fermentation of glucose to ethanol by microbial...
View ArticleDeletion of methylglyoxal synthase gene (mgsA) increased sugar co-metabolism...
AbstractThe use of lignocellulose as a source of sugars for bioproducts requires the development of biocatalysts that maximize product yields by fermenting mixtures of hexose and pentose sugars to...
View ArticleGenetic engineering of Enterobacter asburiae strain JDR-1 for efficient d(−)...
AbstractIn the dilute acid pretreatment of lignocellulose, xylose substituted with α-1,2-methylglucuronate is released as methylglucuronoxylose (MeGAX), which cannot be fermented by biocatalysts...
View ArticleGenetic changes that increase 5-hydroxymethyl furfural resistance in...
AbstractThe ability of a biocatalyst to tolerate furan inhibitors present in hemicellulose hydrolysates is important for the production of renewable chemicals. This study shows EMFR9, a...
View ArticleRemoving chiral contamination of lactate solutions by selective metabolism of...
AbstractObjectiveA bio-based process is appealing for purification of l-lactic acid, the major enantiomer of polylactic acid syrup, generated by thermochemical processes at the end of life of PLA-based...
View ArticleSweet Sorghum Juice and Bagasse as Feedstocks for the Production of Optically...
AbstractSweet sorghum is a bioenergy crop that produces large amounts of soluble sugars in its stems (3–7 Mg ha−1) and generates significant amounts of bagasse (15–20 Mg ha−1) as a lignocellulosic...
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